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DNA定量試劑盒的應用細節

更新時(shí)間：2017-09-06      點(diǎn)擊次數：913
  DNA定量試劑盒靈敏度高，數據可靠，重現性好。操作簡(jiǎn)便，省時(shí)省力。水溶性，不需要換液，尤其適合于懸浮細胞。無(wú)需放射性同位素和有機溶劑，對細胞毒性低。為1瓶溶液，毋需預制，即開(kāi)即用。適合于高通量藥物篩選。
 
  DNA定量試劑盒樣本涵蓋，福爾馬林固定組織樣本(FFPE),其 DNA 損壞程度較重，激光顯微切割捕獲的樣本;提取自流式細胞的 DNA;血漿或血清中的游離 DNA;其他微量或非常珍貴的臨床樣本。
 
  DNA定量試劑盒系統檢測，標準曲線(xiàn)測定1次即可，如果儀器更換，則需要重新測定1次。如果熒光讀數過(guò)高，可以相應稀釋樣品量?？梢允褂脽晒饷笜藘x檢測，試劑用量和體系均除以5，包括標準 DNA 含量，其計算公式[根據標準曲線(xiàn)獲得樣品對應DNA  濃度（微克）X樣品稀釋倍數]÷0.1（樣品容量；毫升）＝微克/毫升。鹽類(lèi)、尿素、乙醇、氯仿、去垢劑、蛋白、瓊脂糖、單鏈 DNA和RNA不會(huì )干擾檢測。
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